HTRA1 Monoclonal antibody, PBS Only

HTRA1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11G12

Cat no : 68053-1-PBS

Synonyms

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

---

Validation Data Gallery

• 

  WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68053-1-Ig (same clone as 68053-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug HTRA1 Monoclonal antibody (68053-1-Ig, Clone:1B11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

---

Product Information

68053-1-PBS targets HTRA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HTRA1 fusion protein Ag31204
• Full Name: HtrA serine peptidase 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: NM_002775
• Gene Symbol: HTRA1
• Gene ID (NCBI): 5654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.