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HTRA1 Monoclonal antibody

HTRA1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B11G12

Cat no : 68053-1-Ig

Synonyms

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HTRA1 Monoclonal antibody (68053-1-Ig)

    WB analysis using 68053-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using HTRA1 Monoclonal antibody (68053-1-Ig)

    WB analysis using 68053-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using HTRA1 Monoclonal antibody (68053-1-Ig)

    FC experiment of HeLa using 68053-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HTRA1 Monoclonal antibody (68053-1-Ig, Clone:1B11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhTERT-RPE1 cells, pig brain tissue, HeLa cells, HepG2 cells, L02 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68053-1-Ig targets HTRA1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HTRA1 fusion protein Ag31204
Full Name HtrA serine peptidase 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberNM_002775
Gene Symbol HTRA1
Gene ID (NCBI) 5654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

Protocols

Product Specific Protocols
WB protocol for HTRA1 antibody 68053-1-IgDownload protocol
FC protocol for HTRA1 antibody 68053-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols