HSP60 Monoclonal antibody, PBS Only

HSP60 Monoclonal Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10E7

Cat no : 66041-1-PBS

Synonyms

60 kDa chaperonin, Chaperonin 60, GROEL, Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1

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Validation Data Gallery

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  WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

  IP result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

  1X10^6 HeLa cells were stained with 0.2ug HSP60 antibody (66041-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66041-1-PBS targets HSP60 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP60 fusion protein Ag7848
• Full Name: heat shock 60kDa protein 1 (chaperonin)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC003030
• Gene Symbol: HSPD1
• Gene ID (NCBI): 3329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)