HSPA2 Monoclonal antibody, PBS Only

HSPA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4A9

Cat no : 66291-1-PBS

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

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Validation Data Gallery

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  WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66291-1-Ig (same clone as 66291-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66291-1-Ig (same clone as 66291-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66291-1-PBS targets HSPA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPA2 fusion protein Ag24228
• Full Name: heat shock 70kDa protein 2
• Calculated Molecular Weight: 639 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC036107
• Gene Symbol: HSPA2
• Gene ID (NCBI): 3306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)