HSPA2 Monoclonal antibody

HSPA2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1A4A9

Cat no : 66291-1-Ig

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

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Validation Data Gallery

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  WB analysis using 66291-1-Ig

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66291-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66291-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 66291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, ROS1728 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human testis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

66291-1-Ig targets HSPA2 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPA2 fusion protein Ag24228
• Full Name: heat shock 70kDa protein 2
• Calculated Molecular Weight: 639 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC036107
• Gene Symbol: HSPA2
• Gene ID (NCBI): 3306
• RRID: AB_2881674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Publications

Species	Application	Title
human	WB	Clin Epigenetics; Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations.; Authors - J Pei
human	WB	Front Cardiovasc Med; Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy.; Authors - Maike Schuldt
human	WB	Cells; Protein Quality Control Activation and Microtubule Remodeling in Hypertrophic Cardiomyopathy.; Authors - Larissa M Dorsch