CoraLite®555-conjugated HSP70 Monoclonal antibody

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  IF Staining of HeLa using CL555-66183

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 HSP70 antibody (CL555-66183, Clone: 4E7E5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66183

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human HSP70 (CL555-66183, Clone:4E7E5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HSP70 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL555-66183

Clone No.4E7E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, yeast

Applications

IF/ICC, FC (Intra)

Synonyms

HSPA1A, 4E7E5, Heat shock 70 kDa protein 1A, heat shock 70kDa protein 1A, Heat shock protein family A member 1A

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66183 targets HSP70 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, yeast samples.

• Tested Reactivity: human, mouse, rat, yeast
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP70 fusion protein Ag19111
• Full Name: heat shock 70kDa protein 1A
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC009322
• Gene Symbol: HSP70
• Gene ID (NCBI): 3303
• RRID: AB_2934669
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P0DMV8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)