CoraLite®594-conjugated HSP27 Monoclonal antibody

HSP27 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E7A1

Cat no : CL594-66767

Synonyms

28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27

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Validation Data Gallery

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  IF Staining of MCF-7 using CL594-66767

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 HSP27 antibody (CL594-66767, Clone: 3E7A1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66767 targets HSP27 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP27 fusion protein Ag27859
• Full Name: heat shock 27kDa protein 1
• Calculated Molecular Weight: 19-23 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC012768
• Gene Symbol: HSPB1
• Gene ID (NCBI): 3315
• RRID: AB_2920032
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).