CoraLite®594-conjugated HRD1/SYVN1 Monoclonal antibody

HRD1/SYVN1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C8A7

Cat no : CL594-67488

Synonyms

HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67488

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated HRD1/SYVN1 antibody (CL594-67488, Clone: 2C8A7 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67488 targets HRD1/SYVN1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HRD1/SYVN1 fusion protein Ag4456
• Full Name: synovial apoptosis inhibitor 1, synoviolin
• Calculated Molecular Weight: 617 aa, 68 kDa
• Observed Molecular Weight: 68-76 kDa
• GenBank Accession Number: BC030530
• Gene Symbol: HRD1
• Gene ID (NCBI): 84447
• RRID: AB_2920146
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).