|Positive WB detected in
|A549 cells, Jurkat cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells
|Positive IHC detected in
|human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:500-1:2000
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
67488-1-Ig targets HRD1/SYVN1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Human, Mouse, Rat
|human, mouse, goat
|Host / Isotype
|Mouse / IgG2b
|HRD1/SYVN1 fusion protein Ag4456
|synovial apoptosis inhibitor 1, synoviolin
|Calculated molecular weight
|617 aa, 68 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).
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