CoraLite® Plus 488-conjugated HPS4 Polyclonal antibody

HPS4 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14627

Synonyms

bK1048E9.4, bK1048E9.5, Hermansky Pudlak syndrome 4, HPS4, KIAA1667, LE, Light ear protein homolog

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-14627

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HPS4 antibody (CL488-14627) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14627 targets HPS4 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPS4 fusion protein Ag6202
• Full Name: Hermansky-Pudlak syndrome 4
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 70-90 kDa
• GenBank Accession Number: BC065030
• Gene Symbol: HPS4
• Gene ID (NCBI): 89781
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Hermansky-Pudlak syndrome (HPS) is a genetic disease characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. HPS1 and HPS4 are the most frequently mutated genes associated with HPS in humans. Both of HPS1 and HPS4 are components of two complexes involved in biogenesis of melanosome and lysosome-related organelles: BLOC-3 and BLOC-4. HPS4 is supposed to interact with HPS1 and stabilize HPS1. The human HPS4 migrates at about 90 kDa on SDS-PAGE, versus its predicated molecular mass of 77 kDa.