Tested Applications
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66798 targets HPGD in IF/ICC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | HPGD fusion protein Ag1499 Predict reactive species |
Full Name | hydroxyprostaglandin dehydrogenase 15-(NAD) |
Calculated Molecular Weight | 29 kDa |
Observed Molecular Weight | 25-28 kDa |
GenBank Accession Number | BC018986 |
Gene Symbol | HPGD |
Gene ID (NCBI) | 3248 |
RRID | AB_2920040 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P15428 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 HPGD antibody CL594-66798 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |