HORMAD1 Recombinant antibody

HORMAD1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241117E3

Cat no : 84003-5-RR

Synonyms

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3

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Validation Data Gallery

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  WB analysis of human testis using 84003-5-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 84003-5-RR (HORMAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 84003-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug HORMAD1 Recombinant antibody (84003-5-RR, Clone:241117E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 84003-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84003-5-RR against Human HORMAD1 were performed. The affinity constant is 2.32 nM.

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84003-5-RR is HORMAD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HORMAD1 fusion protein Ag4914
• Full Name: HORMA domain containing 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC047406
• Gene Symbol: HORMAD1
• Gene ID (NCBI): 84072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].