HORMAD1 Recombinant antibody, PBS Only

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  WB analysis of human testis using 84003-5-RR (same clone as 84003-5-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 84003-5-RR (HORMAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84003-5-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84003-5-RR (same clone as 84003-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug HORMAD1 Recombinant antibody (84003-5-RR, Clone:241117E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84003-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84003-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84003-5-RR against Human HORMAD1 were performed. The affinity constant is 2.32 nM.

HORMAD1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84003-5-PBS

Clone No.241117E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3

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Product Information

84003-5-PBS targets HORMAD1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HORMAD1 fusion protein Ag4914
• Full Name: HORMA domain containing 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC047406
• Gene Symbol: HORMAD1
• Gene ID (NCBI): 84072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q86X24
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].