CoraLite®594-conjugated HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10A5

Cat no : CL594-66743

Synonyms

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

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Validation Data Gallery

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  IF Staining of human liver using CL594-66743

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:100.

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  IF Staining of human liver using CL594-66743

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:100.

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  IF Staining of mouse spleen using CL594-66743

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®594 HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using CL594-66743

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human HO-1/HMOX1 (CL594-66743, Clone:2D10A5) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: mouse spleen tissue, human liver tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66743 targets HO-1/HMOX1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HO-1/HMOX1 fusion protein Ag21296
• Full Name: heme oxygenase (decycling) 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001491
• Gene Symbol: HO-1
• Gene ID (NCBI): 3162
• RRID: AB_2883623
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C