HNRNPC Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HNRNPC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11760-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

  WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  WB analysis using 11760-1-AP (same clone as 11760-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11760-1-AP (same clone as 11760-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11760-1-AP (same clone as 11760-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

  IP result of anti-HNRNPC (IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:10000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11760-1-AP (same clone as 11760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11760-1-AP (HNRNPC antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 11760-1-AP (same clone as 11760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 11760-1-AP (same clone as 11760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (11760-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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Product Information

11760-1-PBS targets HNRNPC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPC fusion protein Ag2356
• Full Name: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa, 36-40 kDa
• GenBank Accession Number: BC003394
• Gene Symbol: HNRNPC
• Gene ID (NCBI): 3183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.