HNRNPA3 Polyclonal antibody

• Featured Product
• KD/KO Validated

HNRNPA3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 25142-1-AP

Synonyms

HNRPA3, HNRNPA 3, hnRNP A3, FBRNP

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 25142-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 25142-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 25142-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 25142-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25142-1-AP (HNRNPA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25142-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 25142-1-AP (HNRNPA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 25142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200, E-cadherin antibody (20874-1-AP, Magenta), CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange).

• 

  IF Staining of HeLa using 25142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, Jurkat cells
• Positive IHC detected in: human stomach tissue, human colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

25142-1-AP targets HNRNPA3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPA3 fusion protein Ag18248
• Full Name: heterogeneous nuclear ribonucleoprotein A3
• Calculated Molecular Weight: 378 aa, 40 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC113470
• Gene Symbol: HNRNPA3
• Gene ID (NCBI): 220988
• RRID: AB_2879921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.

Publications

Species	Application	Title
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An
		Arterioscler Thromb Vasc Biol; Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity.; Authors - Kenneth Stapleton
mouse	IP,IF,IHC	Neurobiol Dis; ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity.; Authors - Haiyan An
human	WB,IF	Sci China Life Sci; Identification of mecciRNAs and their roles in the mitochondrial entry of proteins.; Authors - Xu Liu
mouse	WB	Mediators Inflamm; Integrated Functional Analysis of the Nuclear Proteome of Classically and Alternatively Activated Macrophages.; Authors - John E Wiktorowicz

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tatyana (Verified Customer) (01-21-2023)

Works well in ICC and WB in human cells. Also gives a weak but specific signal in the mouse CNS tissue (spinal cord). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa ICC: 5% goat serum in PBST; mainly nuclear IHC: 4% PFA fixed and paraffinised sections; standard antigen retrieval and DAB detection; mainly nuclear Used at 1:1000, with incubation overnight at 4C, in all applications

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells; mouse / spinal cord

HNRNPA3 Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells; mouse / spinal cord (Cat no:25142-1-AP)