HNRNPA2B1 Recombinant antibody

HNRNPA2B1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240831E3

Cat no : 83773-7-RR

Synonyms

HNRPA2, HNRNPB1, HNRNPA2, hnRNP A2/B1, FLJ22720

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Validation Data Gallery

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  WB analysis using 83773-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83773-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83773-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83773-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (83773-7-RR, Clone: 240831E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83773-7-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPA2B1 Recombinant antibody (83773-7-RR, Clone:240831E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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  Affinity and Kinetic Characterization of 83773-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83773-7-RR against Human HNRNPA2B1 were performed. The affinity constant is 5.04 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Jurkat cells, K-562 cells, HL-60 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83773-7-RR is HNRNPA2B1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNRNPA2B1 fusion protein Ag6563
• Full Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 36 kDa, 38 kDa
• GenBank Accession Number: BC000506
• Gene Symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (38kd) and HNRNPB1 (36kd), by alternative splicing.