HNRNPA2B1 Recombinant antibody, PBS Only

HNRNPA2B1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240831E3

Cat no : 83773-7-PBS

Synonyms

HNRPA2, HNRNPB1, HNRNPA2, hnRNP A2/B1, FLJ22720

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Validation Data Gallery

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  WB analysis using 83773-7-RR (same clone as 83773-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83773-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83773-7-RR (same clone as 83773-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83773-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83773-7-RR (same clone as 83773-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83773-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83773-7-RR (same clone as 83773-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (83773-7-RR, Clone: 240831E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83773-7-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83773-7-RR (same clone as 83773-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPA2B1 Recombinant antibody (83773-7-RR, Clone:240831E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 83773-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83773-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83773-7-RR against Human HNRNPA2B1 were performed. The affinity constant is 5.04 nM.

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Product Information

The immunogen of 83773-7-PBS is HNRNPA2B1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNRNPA2B1 fusion protein Ag6563
• Full Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 36 kDa, 38 kDa
• GenBank Accession Number: BC000506
• Gene Symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (38kd) and HNRNPB1 (36kd), by alternative splicing.