HMMR Monoclonal antibody

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  WB analysis using 68718-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68718-1-Ig (HMMR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68718-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR antibody (68718-1-Ig, Clone: 1C6B5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

HMMR Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68718-1-Ig

Clone No.1C6B5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein, IHABP, CD168

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Ramos cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68718-1-Ig targets HMMR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMMR fusion protein Ag33723
• Full Name: hyaluronan-mediated motility receptor (RHAMM)
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: NM_012484
• Gene Symbol: HMMR
• Gene ID (NCBI): 3161
• RRID: AB_3670411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)