HMGB2 Recombinant antibody, PBS Only

HMGB2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240453H5

Cat no : 83482-5-PBS

Synonyms

HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2

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Validation Data Gallery

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  IF Staining of HepG2 using 83482-5-RR (same clone as 83482-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGB2 antibody (83482-5-RR, Clone: 240453H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83482-5-RR (same clone as 83482-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-5-RR, Clone:240453H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83482-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83482-5-RR against Human HMGB2 were performed. The affinity constant is 44.4 nM.

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Product Information

The immunogen of 83482-5-PBS is HMGB2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HMGB2 fusion protein Ag6135
• Full Name: high-mobility group box 2
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: BC001063
• Gene Symbol: HMGB2
• Gene ID (NCBI): 3148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.