HMGB1 Recombinant antibody, PBS Only (Capture)

HMGB1 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230182C11

Cat no : 82973-2-PBS

Synonyms

HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1

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Validation Data Gallery

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  WB analysis using 82973-2-RR (same clone as 82973-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82973-2-RR (HMGB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82973-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00311-2

  Sandwich ELISA standard curve of MP00311-2, HMGB1 Recombinant Matched Antibody Pair, PBS Only. 82973-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1264. 82973-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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Product Information

82973-2-PBS targets HMGB1 as part of a matched antibody pair:

MP00311-2: 82973-2-PBS capture and 82973-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HMGB1 fusion protein Ag1264
• Full Name: high-mobility group box 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC003378
• Gene Symbol: HMGB1
• Gene ID (NCBI): 3146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.