HMGB1 Recombinant Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Rabbit / IgG
Detection: Rabbit / IgG
Reactivity
Capture: human
Detection: human
Applications
Sandwich ELISA
Range
Sandwich ELISA: 0.313-20 ng/mL
Validation Data Gallery
Tested Applications
Sandwich ELISA
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP00311-2 targets HMGB1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.
Range: 0.313-20 ng/mL (Sandwich ELISA);
Capture antibody: HMGB1 Recombinant antibody, PBS Only (Capture) 82973-2-PBS (230182C11). 100 μg. Concentration 1 mgl/ml.
Detection antibody: HMGB1 Recombinant antibody, PBS Only (Detector) 82973-3-PBS (230182C2). 100 μg. Concentration 1 mgl/ml.
Alternative HMGB1 matched antibody pairs: MP00311-1
Matched pair protein standard: Ag1264
Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 82973-2-PBS |
| Tested Reactivity | human |
| Immunogen | HMGB1 fusion protein Ag1264 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | high-mobility group box 1 |
| Synonyms | HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1 |
| GenBank accession number | BC003378 |
| Gene symbol | HMGB1 |
| Uniprot | P09429 |
| Gene ID (NCBI) | 3146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 82973-3-PBS |
| Tested Reactivity | human |
| Immunogen | HMGB1 fusion protein Ag1264 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | high-mobility group box 1 |
| Synonyms | HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1 |
| GenBank accession number | BC003378 |
| Gene symbol | HMGB1 |
| Uniprot | P09429 |
| Gene ID (NCBI) | 3146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |