HMGA1 Polyclonal antibody, PBS Only

HMGA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29895-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29895-1-AP (same clone as 29895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29895-1-AP (HMGA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29895-1-AP (same clone as 29895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

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Product Information

29895-1-PBS targets HMGA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGA1 fusion protein Ag31866
• Full Name: high mobility group AT-hook 1
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC071864
• Gene Symbol: HMGA1
• Gene ID (NCBI): 3159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).