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HMGA1 Polyclonal antibody

HMGA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29895-1-AP

Synonyms

HMG-I(Y), HMGA1A, High mobility group protein R, High mobility group protein HMG-I/HMG-Y, High mobility group protein A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMGA1 Polyclonal antibody (29895-1-AP)

    WB analysis using 29895-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29895-1-AP (HMGA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human breast cancer using 29895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human prostate cancer using 29895-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human prostate cancer using 29895-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human pancreas cancer using 29895-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human pancreas cancer using 29895-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, MKN-45 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29895-1-AP targets HMGA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGA1 fusion protein Ag31866
Full Name high mobility group AT-hook 1
Observed Molecular Weight20 kDa
GenBank Accession NumberBC071864
Gene Symbol HMGA1
Gene ID (NCBI) 3159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).

Protocols

Product Specific Protocols
WB protocol for HMGA1 antibody 29895-1-APDownload protocol
IHC protocol for HMGA1 antibody 29895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pathol Oncol Res

Long non-coding RNA MYU promotes ovarian cancer cell proliferation by sponging miR-6827-5p and upregulating HMGA1

Authors - Shaoyu Wang
View Article
humanIHC

J Thorac Dis

Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients

Authors - Zhanming Ma
View Article