HLA class I ABC Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HLA class I ABC Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C5B7

Cat no : 66013-1-PBS

Synonyms

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3

---

Validation Data Gallery

• 

  WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 66013-1-Ig (same clone as 66013-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human white blood using 66013-1-Ig (same clone as 66013-1-PBS)

  human white blood cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66013-1-Ig (same clone as 66013-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66013-1-Ig (same clone as 66013-1-PBS)

  pig spleen tissue was subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66013-1-Ig (same clone as 66013-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66013-1-Ig (same clone as 66013-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

  1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66013-1-PBS targets HLA class I ABC in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HLA class I ABC fusion protein Ag7488
• Full Name: major histocompatibility complex, class I, A
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC003069
• Gene Symbol: HLA-A
• Gene ID (NCBI): 3105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)