CoraLite® Plus 488-conjugated HLA-F Monoclonal antibody

HLA-F Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12C2

Cat no : CL488-66819

Synonyms

HLA F antigen, HLA F, HLA CDA12, HLA 5.4, CDA12

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Validation Data Gallery

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  IF Staining of Raji using CL488-66819

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 HLA-F antibody (CL488-66819, Clone: 1B12C2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66819 targets HLA-F in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HLA-F fusion protein Ag6475
• Full Name: major histocompatibility complex, class I, F
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC062991
• Gene Symbol: HLA-F
• Gene ID (NCBI): 3134
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)