CoraLite® Plus 647 Anti-Human HLA-DR (L243) Rabbit Recombinant Antibody

HLA-DR Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

L243

Cat no : CL647-65503

Synonyms

HLA DRA, HLA DRA1, HLA-DRA

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65503

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human HLA-DR Rabbit Recombinant Antibody (CL647-65503, Clone:L243) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65503

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human HLA-DR Rabbit Recombinant Antibody (CL647-65503, Clone:L243) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65503

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human HLA-DR Rabbit Recombinant Antibody (CL647-65503, Clone:L243) (left) or 5 ul CoraLite® Plus 647 Anti-Human HLA-DR Mouse Monoclonal Antibody (CL647-65218, Clone:L243) (right). Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65503 targets HLA-DR in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.