HLA-DMA Recombinant antibody

HLA-DMA Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230155E7

Cat no : 82899-2-RR

Synonyms

D6S222E, DAMA 147C13.1 001, DAMC 226H3.5 001, DAQB 96B10.1 001, DASS 67H14.8 001, DMA, DMA protein, hCG_2001572, HLA DMA, HLA DMA protein, HLADM, HLA-DMA, RING6, XXbac BCX136A3.1 001, XXbac BPG181M17.3 001

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Validation Data Gallery

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  WB analysis using 82899-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82899-2-RR (HLA-DMA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 82899-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 82899-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, Ramos cells, Raji cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82899-2-RR targets HLA-DMA in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DMA fusion protein Ag2452
• Full Name: major histocompatibility complex, class II, DM alpha
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC026279
• Gene Symbol: HLA-DMA
• Gene ID (NCBI): 3108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.