HLA-DMA Recombinant antibody, PBS Only

HLA-DMA Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

230155E7

Cat no : 82899-2-PBS

Synonyms

hCG_2001572, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha, DMA protein, DMA, D6S222E

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Validation Data Gallery

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  WB analysis using 82899-2-RR (same clone as 82899-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82899-2-RR (HLA-DMA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82899-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82899-2-RR (same clone as 82899-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82899-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82899-2-RR (same clone as 82899-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82899-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82899-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82899-2-RR against Human HLA-DMA were performed. The affinity constant is 0.303 nM.

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Product Information

82899-2-PBS targets HLA-DMA in Indirect ELISA, IHC, WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DMA fusion protein Ag2452
• Full Name: major histocompatibility complex, class II, DM alpha
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC026279
• Gene Symbol: HLA-DMA
• Gene ID (NCBI): 3108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.