HK3 Monoclonal antibody, PBS Only

HK3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3H3C7

Cat no : 67803-1-PBS

Synonyms

Hexokinase-C, Hexokinase-3, Hexokinase type III, hexokinase 3 (white cell), Hexokinase 3

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Validation Data Gallery

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  WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HK3 antibody (67803-1-Ig, Clone: 3H3C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67803-1-Ig (same clone as 67803-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug HK3 Monoclonal antibody (67803-1-Ig, Clone:3H3C7) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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Product Information

67803-1-PBS targets HK3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HK3 fusion protein Ag30798
• Full Name: hexokinase 3 (white cell)
• Calculated Molecular Weight: 923 aa, 99 kDa
• Observed Molecular Weight: 99 kDa
• GenBank Accession Number: BC028129
• Gene Symbol: HK3
• Gene ID (NCBI): 3101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.