HIP14; ZDHHC17 Polyclonal antibody, PBS Only

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  WB analysis using 30022-1-AP (same clone as 30022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30022-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30022-1-AP (same clone as 30022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30022-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 30022-1-AP (same clone as 30022-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HIP14; ZDHHC17 antibody (30022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30022-1-PBS in a different storage buffer formulation.

HIP14; ZDHHC17 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 30022-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ZDHHC17, HIP 14, EC:2.3.1.225, EC:2.3.1.-, DHHC domain-containing cysteine-rich protein 17

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Product Information

30022-1-PBS targets HIP14; ZDHHC17 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIP14; ZDHHC17 fusion protein Ag32654
• Full Name: zinc finger, DHHC-type containing 17
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050324
• Gene Symbol: ZDHHC17
• Gene ID (NCBI): 23390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IUH5
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ZDHHC17 is a neuronal palmitoyl transferase. Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.