HIP14; ZDHHC17 Polyclonal antibody

• Featured Product
• KD/KO Validated

HIP14; ZDHHC17 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30022-1-AP

Synonyms

DHHC 17, HIP 14, HIP 3, HIP14, HIP3, HSPC294, Huntingtin yeast partner H, HYPH, KIAA0946, Palmitoyltransferase ZDHHC17, ZDHHC17

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Validation Data Gallery

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  WB analysis using 30022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 30022-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HIP14; ZDHHC17 antibody (30022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, Raji cells, U-251 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30022-1-AP targets HIP14; ZDHHC17 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIP14; ZDHHC17 fusion protein Ag32654
• Full Name: zinc finger, DHHC-type containing 17
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050324
• Gene Symbol: ZDHHC17
• Gene ID (NCBI): 23390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZDHHC17 is a neuronal palmitoyl transferase. Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.