CoraLite® Plus 488-conjugated HIP1 Monoclonal antibody

HIP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D3G3

Cat no : CL488-67163

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

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Validation Data Gallery

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  IF Staining of A549 using CL488-67163

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 HIP1 antibody (CL488-67163, Clone: 3D3G3 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67163 targets HIP1 in IF/ICC applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIP1 fusion protein Ag17982
• Full Name: huntingtin interacting protein 1
• Calculated Molecular Weight: 1037 aa, 116 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC110545
• Gene Symbol: HIP1
• Gene ID (NCBI): 3092
• RRID: AB_2919431
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.