CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody

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  IF Staining of U2OS using CL488-68231

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 HIGD1A antibody (CL488-68231, Clone: 3A8E2 ) at dilution of 1:400.

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  FC experiment of U2OS using CL488-68231

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody (CL488-68231, Clone:3A8E2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HIGD1A Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-68231

Clone No.3A8E2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68231 targets HIGD1A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIGD1A fusion protein Ag14027
• Full Name: HIG1 hypoxia inducible domain family, member 1A
• Calculated Molecular Weight: 93 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC000601
• Gene Symbol: HIGD1A
• Gene ID (NCBI): 25994
• RRID: AB_3672981
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y241
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.