- Featured Product
- KD/KO Validated
CoraLite®595-conjugated HIF1a Polyclonal antibody
HIF1a Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
CoraLite®595 Fluorescent Dye
Cat no : CL595-20960
|Positive FC detected in||HeLa cells|
|Sample-dependent, check data in validation data gallery|
The immunogen of CL595-20960 is HIF1a Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HIF1a fusion protein Ag15198|
|Full Name||hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|
|Calculated molecular weight||826 aa, 93 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC012527|
|Gene ID (NCBI)||3091|
|Conjugate||CoraLite®595 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||592 nm / 611 nm|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Davide (Verified Customer) (10-10-2022)
The antibody performs as its non conjugated counterpart, delivering a staining signal from the cytoplasm but not from the nuclei in positive controls (hypoxic). It is possible to see a slight accumulation of the antibody around the nuclei but it seems to not be able to penetrate inside.