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CoraLite®595-conjugated HIF1a Polyclonal antibody

HIF1a Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®595 Fluorescent Dye

Cat no : CL595-20960

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, Member of PAS protein 1, MOP1, PASD8



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL595-20960 is HIF1a Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF1a fusion protein Ag15198
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight 826 aa, 93 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC012527
Gene symbol HIF1A
Gene ID (NCBI) 3091
Conjugate CoraLite®595 Fluorescent Dye
Excitation/Emission maxima wavelengths592 nm / 611 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Protocols

Product Specific Protocols
FC protocol for CL595 HIF1a antibody CL595-20960Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Davide (Verified Customer) (10-10-2022)

The antibody performs as its non conjugated counterpart, delivering a staining signal from the cytoplasm but not from the nuclei in positive controls (hypoxic). It is possible to see a slight accumulation of the antibody around the nuclei but it seems to not be able to penetrate inside.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
CL595 HIF1a Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:CL595-20960)