HIF-1 alpha Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HIF-1 alpha Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H3C12

Cat no : 66730-1-PBS

Synonyms

HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein

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Validation Data Gallery

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  WB analysis of HeLa using 66730-1-Ig (same clone as 66730-1-PBS)

  Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control. This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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Samples need to be treated with hypoxia stimulation.

Product Information

66730-1-PBS targets HIF-1 alpha in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIF-1 alpha fusion protein Ag15198
• Full Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Calculated Molecular Weight: 826 aa, 93 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC012527
• Gene Symbol: HIF1A
• Gene ID (NCBI): 3091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .