HIF-1 alpha Monoclonal antibody

• Featured Product
• KD/KO Validated

HIF-1 alpha Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

53

CloneNo.

1H3C12

Reviews (3)

Cat no : 66730-1-Ig

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF 1α, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF1α, Member of PAS protein 1, MOP1, PASD8

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Validation Data Gallery

• 

  WB analysis of HeLa using 66730-1-Ig

  Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control.

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Tested Applications

• Positive WB detected in: HeLa cells, Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells

Samples need to be treated with hypoxia stimulation.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 42 publications below
• IHC: See 13 publications below
• IF: See 12 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

66730-1-Ig targets HIF-1 alpha in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIF-1 alpha fusion protein Ag15198
• Full Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Calculated Molecular Weight: 826 aa, 93 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC012527
• Gene Symbol: HIF1A
• Gene ID (NCBI): 3091
• RRID: AB_2882080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Publications

Species	Application	Title
human	WB,IHC,IF,IP	Adv Sci (Weinh); Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation; Authors - Huan Li
	IF	Cell Rep Med; Ultrasound-visible engineered bacteria for tumor chemo-immunotherapy; Authors - Yaozhang Yang
human	WB	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
human	WB	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
human	WB	Oxid Med Cell Longev; Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.; Authors - Ziwei Yang
human	WB	J Med Chem; Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2.; Authors - Xuan Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (05-16-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

HIF-1 alpha Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:66730-1-Ig)

FH

balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: renal Cancer cell line

FH

Diane (Verified Customer) (02-02-2021)

incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1250
• Cell Tissue Type: Cell lysates glioblastoma primary cells

HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig)