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HEK 293 cells Polyclonal antibody

HEK 293 cells Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27347-1-AP

Synonyms

293, HEK 293 cells, HEK293

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using HEK 293 cells Polyclonal antibody (27347-1-AP)

    IF Staining of HEK-293 using 27347-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 27347-1-AP (HEK 293 cells antibody) at dilution of 1:1000 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27347-1-AP targets HEK 293 cells in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cell Lysate
Full Name HEK 293 cells
Gene Symbol
Gene ID (NCBI)
RRIDAB_2880850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Embryonic Kidney 293 cells, also often referred to as HEK 293, 293 cells, or less precisely as HEK cells are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK 293 cells were generated in early 70s by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex Van der Eb's laboratory in Leiden, Holland. This antibody is designed to detect the protein of the HEK293 cells.

Protocols

Product Specific Protocols
IF protocol for HEK 293 cells antibody 27347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Western blotting analysis of proteins separated by agarose native gel electrophoresis.

Authors - Chiaki Sakuma
View Article