HEC1/HEC/NDC80 Recombinant antibody, PBS Only

HEC1/HEC/NDC80 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5A2

Cat no : 83859-1-PBS

Synonyms

NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

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Validation Data Gallery

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  WB analysis using 83859-1-RR (same clone as 83859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83859-1-RR (HEC1/HEC/NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83859-1-PBS is HEC1/HEC/NDC80 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: /
• Gene Symbol: NDC80
• Gene ID (NCBI): 10403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.