HDGF Monoclonal antibody, PBS Only

HDGF Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A7G3

Cat no : 60064-1-PBS

Synonyms

HDGF, Hepatoma derived growth factor, HMG 1L2, HMG1L2

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 60064-1-Ig (same clone as 60064-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60064-1-Ig (same clone as 60064-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60064-1-Ig (same clone as 60064-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60064-1-Ig (same clone as 60064-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 60064-1-Ig (same clone as 60064-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60064-1-PBS targets HDGF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDGF fusion protein Ag1893
• Full Name: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC018991
• Gene Symbol: HDGF
• Gene ID (NCBI): 3068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)