Tested Applications
| Positive WB detected in | SMMC-7721 cells, A549 cells, HeLa cells, HepG2 cells, L02 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
60064-1-Ig targets HDGF in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1893 Product name: Recombinant human HDGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC018991 Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL Predict reactive species |
| Full Name | hepatoma-derived growth factor (high-mobility group protein 1-like) |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC018991 |
| Gene Symbol | HDGF |
| Gene ID (NCBI) | 3068 |
| RRID | AB_2117098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P51858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HDGF antibody 60064-1-Ig | Download protocol |
| WB protocol for HDGF antibody 60064-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs. | ||
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma. | ||
Neural Plast Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy. | ||
Biochem Biophys Res Commun Irradiated fibroblasts promote epithelial-mesenchymal transition and HDGF expression of esophageal squamous cell carcinoma. | ||
Med Oncol The expression of hepatoma-derived growth factor in primary central nervous system lymphoma and its correlation with angiogenesis, proliferation and clinical outcome. | ||
Mol Vis The regulatory role of hepatoma-derived growth factor as an angiogenic factor in the eye. |
