HDAC8 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HDAC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17548-1-PBS

Synonyms

Protein decrotonylase HDAC8, Protein deacetylase HDAC8, HDAC 8, EC:3.5.1.98, EC:3.5.1.-

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Validation Data Gallery

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  WB analysis of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 17548-1-AP (same clone as 17548-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 17548-1-AP (same clone as 17548-1-PBS)

  IP result of anti-HDAC8 (IP:17548-1-AP, 4ug; Detection:17548-1-AP 1:600) with Raji cells lysate 2800ug. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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Product Information

17548-1-PBS targets HDAC8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC8 fusion protein Ag11696
• Full Name: histone deacetylase 8
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC050433
• Gene Symbol: HDAC8
• Gene ID (NCBI): 55869
• RRID: AB_2116926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.