HDAC8 Polyclonal antibody

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  WB analysis using 17548-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 17548-1-AP

  IP result of anti-HDAC8 (IP:17548-1-AP, 4ug; Detection:17548-1-AP 1:600) with Raji cells lysate 2800ug.

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  IHC staining of human lung cancer using 17548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17548-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HDAC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17548-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Protein decrotonylase HDAC8, Protein deacetylase HDAC8, HDAC 8, EC:3.5.1.98, EC:3.5.1.-

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 2 publications below

Product Information

17548-1-AP targets HDAC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC8 fusion protein Ag11696
• Full Name: histone deacetylase 8
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC050433
• Gene Symbol: HDAC8
• Gene ID (NCBI): 55869
• RRID: AB_2116926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY41
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

Publications

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
mouse, rat	WB	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	WB	Oncotarget; Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair.; Authors - Pei Chen
human	WB	Oncogenesis; USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.; Authors - Wei Zhan
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan