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- KD/KO Validated
HDAC8 Polyclonal antibody
HDAC8 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 17548-1-AP
|Positive WB detected in||HeLa cells, Raji cells|
|Positive IP detected in||Raji cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 18 publications below|
|IHC||See 1 publications below|
17548-1-AP targets HDAC8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HDAC8 fusion protein Ag11696|
|Full Name||histone deacetylase 8|
|Calculated molecular weight||377 aa, 42 kDa|
|Observed molecular weight||49 kDa|
|GenBank accession number||BC050433|
|Gene ID (NCBI)||55869|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.
|Product Specific Protocols|
|WB protocol for HDAC8 antibody 17548-1-AP||Download protocol|
|IF protocol for HDAC8 antibody 17548-1-AP||Download protocol|
|IP protocol for HDAC8 antibody 17548-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.
Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.
Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.
Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair.
USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.
Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1.