HDAC5-specific Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HDAC5-specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16166-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 16166-1-AP (same clone as 16166-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16166-1-AP (same clone as 16166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16166-1-AP (same clone as 16166-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 16166-1-AP (same clone as 16166-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16166-1-AP (same clone as 16166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16166-1-AP (same clone as 16166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC5-specific antibody (16166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16166-1-PBS in a different storage buffer formulation.

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Product Information

16166-1-PBS targets HDAC5-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone deacetylase 5
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: BC051824
• Gene Symbol: HDAC5
• Gene ID (NCBI): 10014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone acetylation and deacetylation alternately expose and occlude DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.