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  • KD/KO Validated

HDAC5-specific Polyclonal antibody

HDAC5-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16166-1-AP

Synonyms

Antigen NY CO 9, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9



Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman brain tissue, human heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16166-1-AP targets HDAC5-specific in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name histone deacetylase 5
Calculated molecular weight 122 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC051824
Gene symbol HDAC5
Gene ID (NCBI) 10014
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.

Protocols

Product Specific Protocols
WB protocol for HDAC5-specific antibody 16166-1-APDownload protocol
IHC protocol for HDAC5-specific antibody 16166-1-APDownload protocol
IF protocol for HDAC5-specific antibody 16166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

FASEB J

STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy.

Authors - Wei Du
  • KD Validated
humanWB

J Cell Mol Med

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Authors - Shoujie Feng
  • KD Validated
ratWB, IHC

Cardiovasc Toxicol

Antithetical Regulation of α-Myosin Heavy Chain Between Fetal and Adult Heart Failure Though Shuttling of HDAC5 Regulating YY-1 Function.

Authors - Jie Fang
humanWB

Cell Death Dis

WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.

Authors - Yingtong Feng
mouseWB,CoIP

Int J Mol Med

EGCG prevents pressure overload‑induced myocardial remodeling by downregulating overexpression of HDAC5 in mice.

Authors - Xiao Han
humanWB,CoIP

Chem Biol Interact

microRNA-671-5p reduces tumorigenicity of ovarian cancer via suppressing HDAC5 and HIF-1α expression.

Authors - Dongxian Peng
  • KD Validated