HDAC5 Monoclonal antibody, PBS Only

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  WB analysis using 68437-1-Ig (same clone as 68437-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68437-1-Ig (HDAC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68437-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68437-1-Ig (same clone as 68437-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 68437-1-Ig (HDAC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68437-1-PBS in a different storage buffer formulation.

HDAC5 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68437-1-PBS

Clone No.1B9E6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Antigen NY CO 9, FLJ90614, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9

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Product Information

68437-1-PBS targets HDAC5 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC5 fusion protein Ag30557
• Full Name: histone deacetylase 5
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_005474
• Gene Symbol: HDAC5
• Gene ID (NCBI): 10014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UQL6
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.