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HDAC5 Monoclonal antibody

HDAC5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B9E6

Cat no : 68437-1-Ig

Synonyms

Antigen NY CO 9, FLJ90614, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC5 Monoclonal antibody (68437-1-Ig)

    WB analysis using 68437-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68437-1-Ig (HDAC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HDAC5 Monoclonal antibody (68437-1-Ig)

    WB analysis using 68437-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 68437-1-Ig (HDAC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSaos-2 cells, COLO 320 cells, HeLa cells, T-47D cells, SK-BR-3 cells, A2780 cells, HepG2 cells, HSC-T6 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68437-1-Ig targets HDAC5 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HDAC5 fusion protein Ag30557
Full Name histone deacetylase 5
Calculated Molecular Weight 122 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_005474
Gene Symbol HDAC5
Gene ID (NCBI) 10014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.

Protocols

Product Specific Protocols
WB protocol for HDAC5 antibody 68437-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols