HDAC11 Monoclonal antibody, PBS Only

HDAC11 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12B5

Cat no : 67949-1-PBS

Synonyms

FLJ22237, HD11, HDAC11, histone deacetylase 11

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Validation Data Gallery

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  WB analysis using 67949-1-Ig (same clone as 67949-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 67949-1-Ig (same clone as 67949-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67949-1-Ig (same clone as 67949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67949-1-Ig (same clone as 67949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67949-1-Ig (same clone as 67949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67949-1-Ig (same clone as 67949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67949-1-Ig (same clone as 67949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67949-1-Ig (same clone as 67949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67949-1-PBS in a different storage buffer formulation.

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Product Information

67949-1-PBS targets HDAC11 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC11 fusion protein Ag28335
• Full Name: histone deacetylase 11
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC009676
• Gene Symbol: HDAC11
• Gene ID (NCBI): 79885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC11 belongs to Class IV HDACs. HDAC11 is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. HDAC11 has 2 isoforms with the molecular mass of 33 and 39 kDa.