HDAC11 Monoclonal antibody

HDAC11 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G12B5

Cat no : 67949-1-Ig

Synonyms

FLJ22237, HD11, HDAC11, histone deacetylase 11

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Validation Data Gallery

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  WB analysis using 67949-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 67949-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, SKOV-3 cells, T-47D cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

67949-1-Ig targets HDAC11 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC11 fusion protein Ag28335
• Full Name: histone deacetylase 11
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC009676
• Gene Symbol: HDAC11
• Gene ID (NCBI): 79885
• RRID: AB_2918701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC11 belongs to Class IV HDACs. HDAC11 is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. HDAC11 has 2 isoforms with the molecular mass of 33 and 39 kDa.

Publications

Species	Application	Title
human	IHC	Transl Lung Cancer Res; Clinicopathological characteristics and prognostic significance of HDAC11 protein expression in non-small cell lung cancer: a retrospective study.; Authors - Di Lu
human	WB	Cell Discov; Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance; Authors - Yan He
human	WB	J South Med Univ; [Overexpression of histone deacetylase 11 suppresses basal-like breast cancer cell invasion and metastasis]; Authors - Zhang Yi ; KD Validated
human,mouse	WB	iScience; Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication; Authors - Shengli Ming