HBS1L Monoclonal antibody, PBS Only

HBS1L Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10C4

Cat no : 68146-1-PBS

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

---

Validation Data Gallery

• 

  WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68146-1-Ig (same clone as 68146-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68146-1-Ig (same clone as 68146-1-PBS)

  WB result of HBS1L antibody (68146-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

---

Product Information

68146-1-PBS targets HBS1L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HBS1L fusion protein Ag0388
• Full Name: HBS1-like (S. cerevisiae)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001465
• Gene Symbol: HBS1L
• Gene ID (NCBI): 10767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.